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human intestinal epithelial cells  (ATCC)


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    ATCC human intestinal epithelial cells
    Human Intestinal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+intestinal+caco+2+cells+htb+37/Caco-2/pm42171256-57-0-5
    Average 99 stars, based on 15185 article reviews
    human intestinal epithelial cells - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Partially hydrolyzed guar gum upregulates heat shock protein 27 in intestinal Caco-2 cells and mouse intestine via mTOR and ERK signaling.
    Article Snippet: BACKGROUND: The intestinal epithelium acts as a barrier against harmful luminal materials, thus preventing intestinal diseases andmaintaining intestinal health.. Heat shock protein 27 (HSP27) promotes intestinal epithelial integrity under both physiological and stressed conditions.. The effects of partially hydrolyzed guar gum (PHGG) on HSP27 expression in intestinal Caco-2 cells and mouse intestines were investigated.

    Article Title: ALOX15‐Derived Oxylipins Attenuate Macrophage Inflammatory Signaling Via a Gα q –PLC–PKC Pathway
    Article Snippet: Murine macrophage Raw264.7 cells (TIB‐71) and human intestinal Caco‐2 cells (HTB‐37) were purchased from American Type Culture Collection (Mannasas, VA, USA).

    Article Title: N-butyrate increases heat shock protein 70 through heat shock factor 1 and AMP-activated protein kinase pathways in human intestinal Caco-2 cells.
    Article Snippet: Impaired integrity of the intestinal epithelium is a cause of intestinal and extraintestinal diseases.. Heat shock protein 70 (HSP70), a cytoprotective protein, plays an important role in maintaining intestinal homeostasis.. The intestinal expression of HSP70 is linked with the local microbiota.

    Modification:

    Article Title: 5,7-Dimethoxyflavone enhances barrier function by increasing occludin and reducing claudin-2 in human intestinal Caco-2 cells
    Article Snippet: All other chemicals were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). .. Human intestinal Caco-2 cells (HTB-37) were purchased from American Type Culture Collection (Manassas, VA, USA) and propagated and maintained in a high-glucose (4.5 g/L D-glucose) Dulbecco’s modified Eagle’s medium supplemented with 100 mL/L decomplemented fetal bovine serum, 44 mM sodium bicarbonate, 1 mM sodium pyruvate, 50 000 U/L penicillin, and 50 mg/L streptomycin (Noda et al., 2013; Suzuki & Hara, 2009). .. The cells were seeded into permeable polyester membrane filter supports (Transwell, 12-mm diameter, 0.4- μm pore size; Corning, NY, USA) at a density of 0.25 × 106 cells/cm2.

    Cell Culture:

    Article Title: Bioactive compounds of Citrus hystrix peel ethanolic extract and their antioxidant potential under hydrogen peroxide-induced oxidative stress in Caco-2 cells
    Article Snippet: .. Human intestinal Caco-2 cells (HTB-37) were purchased from American Type Culture Collection (Manassas, VA, USA) and maintained under standard cell culture conditions (ATCC, 2021). ..



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    Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

    Journal: Nutrients

    Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

    doi: 10.3390/nu18091393

    Figure Lengend Snippet: Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

    Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

    Techniques: Viability Assay, Control

    Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

    Journal: Nutrients

    Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

    doi: 10.3390/nu18091393

    Figure Lengend Snippet: Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

    Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

    Techniques: Concentration Assay, Control

    Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

    Journal: Nutrients

    Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

    doi: 10.3390/nu18091393

    Figure Lengend Snippet: Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

    Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

    Techniques: Permeability, Enzyme-linked Immunosorbent Assay, Derivative Assay, Control

    The percentage viability of Caco-2 cells follow 48 h exposure to AA-7 ( a ) and glucose uptake inhibition kinetics with fluorescent glucose 2-NBDG, following treatment with AA-7 ( b ). Data are presented as mean ± SD for three or more independent trials with consistent findings. The use of different letters (a-c) identifies means which exhibit significant differences ( p < 0.05).

    Journal: Scientific Reports

    Article Title: Bee pollen-derived peptide with dual DPP-IV Inhibition and glucose transport modulation

    doi: 10.1038/s41598-026-39009-1

    Figure Lengend Snippet: The percentage viability of Caco-2 cells follow 48 h exposure to AA-7 ( a ) and glucose uptake inhibition kinetics with fluorescent glucose 2-NBDG, following treatment with AA-7 ( b ). Data are presented as mean ± SD for three or more independent trials with consistent findings. The use of different letters (a-c) identifies means which exhibit significant differences ( p < 0.05).

    Article Snippet: Human intestinal epithelial cell line Caco-2 cells (ATCC ® HTB-37TM) were supplied by the American Type Culture Collection (ATCC, Manassas, VA, USA), before being cultured in Eagle’s Minimum Essential Medium (EMEM) which was supplemented using L-glutamine and 10% fetal bovine serum (Gibco, Rockville, MD, USA).

    Techniques: Inhibition